Plasma glutaredoxin activity in healthy subjects and patients with abnormal glucose levels or overt type 2 diabetes.
Identifieur interne : 000628 ( Main/Exploration ); précédent : 000627; suivant : 000629Plasma glutaredoxin activity in healthy subjects and patients with abnormal glucose levels or overt type 2 diabetes.
Auteurs : Yatao Du [Suède] ; Huihui Zhang ; Sergio Montano ; Jesper Hegestam ; Neda Rajamand Ekberg ; Arne Holmgren ; Kerstin Brismar ; Johanna S. UngerstedtSource :
- Acta diabetologica [ 1432-5233 ] ; 2014.
Descripteurs français
- KwdFr :
- Adulte (MeSH), Diabète de type 2 (sang), Femelle (MeSH), Glutarédoxines (sang), Humains (MeSH), Hyperglycémie provoquée (MeSH), Intolérance au glucose (sang), Jeune adulte (MeSH), Mâle (MeSH), Oxydoréduction (MeSH), Spectrométrie de fluorescence (MeSH), Statistique non paramétrique (MeSH), Stress oxydatif (physiologie), Technique de Western (MeSH).
- MESH :
English descriptors
- KwdEn :
- Adult (MeSH), Blotting, Western (MeSH), Diabetes Mellitus, Type 2 (blood), Female (MeSH), Glucose Intolerance (blood), Glucose Tolerance Test (MeSH), Glutaredoxins (blood), Humans (MeSH), Male (MeSH), Oxidation-Reduction (MeSH), Oxidative Stress (physiology), Spectrometry, Fluorescence (MeSH), Statistics, Nonparametric (MeSH), Young Adult (MeSH).
- MESH :
- chemical , blood : Glutaredoxins.
- blood : Diabetes Mellitus, Type 2, Glucose Intolerance.
- physiology : Oxidative Stress.
- Adult, Blotting, Western, Female, Glucose Tolerance Test, Humans, Male, Oxidation-Reduction, Spectrometry, Fluorescence, Statistics, Nonparametric, Young Adult.
Abstract
Oxidative stress induced by hyperglycemia is a key factor in the pathogenesis of diabetes complications. Glutaredoxin 1(Grx1) is a cytosolic redox protein that catalyzes GSH-dependent thiol redox reactions and reversible protein S-glutathionylation. In humans, Grx1 antigen has previously been detected in plasma; however, it has hitherto been unclear if plasma Grx1 is enzymatically active, which would indicate an extracellular function of the protein. Given that glucose overload damages cells through oxidative stress responses, we investigated whether postprandial hyperglycemia induces changes in extracellular Grx1 in patients with abnormal glucose tolerance and healthy subjects. Using a novel sensitive fluorescent substrate assay, we demonstrated that plasma Grx consists of active protein. Grx antigen, activity and total antioxidant capacity were significantly elevated in patients compared to healthy subjects. In response to oral glucose tolerance test, Grx activity and antioxidant capacity increased significantly in healthy volunteers, however, not to the high levels of the patients. In conclusion, these results indicate an extracellular function of plasma Grx in blood glucose metabolism. Thus, Grx may be a marker of increased oxidative stress during hyperglycemia in healthy subjects and may be a risk marker of progression toward diabetes onset.
DOI: 10.1007/s00592-013-0498-2
PubMed: 23836328
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Ungerstedt</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:23836328</idno><idno type="pmid">23836328</idno><idno type="doi">10.1007/s00592-013-0498-2</idno><idno type="wicri:Area/Main/Corpus">000728</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000728</idno><idno type="wicri:Area/Main/Curation">000728</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000728</idno><idno type="wicri:Area/Main/Exploration">000728</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Plasma glutaredoxin activity in healthy subjects and patients with abnormal glucose levels or overt type 2 diabetes.</title><author><name sortKey="Du, Yatao" sort="Du, Yatao" uniqKey="Du Y" first="Yatao" last="Du">Yatao Du</name><affiliation wicri:level="3"><nlm:affiliation>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Zhang, Huihui" sort="Zhang, Huihui" uniqKey="Zhang H" first="Huihui" last="Zhang">Huihui Zhang</name></author><author><name sortKey="Montano, Sergio" sort="Montano, Sergio" uniqKey="Montano S" first="Sergio" last="Montano">Sergio Montano</name></author><author><name sortKey="Hegestam, Jesper" sort="Hegestam, Jesper" uniqKey="Hegestam J" first="Jesper" last="Hegestam">Jesper Hegestam</name></author><author><name sortKey="Ekberg, Neda Rajamand" sort="Ekberg, Neda Rajamand" uniqKey="Ekberg N" first="Neda Rajamand" last="Ekberg">Neda Rajamand Ekberg</name></author><author><name sortKey="Holmgren, Arne" sort="Holmgren, Arne" uniqKey="Holmgren A" first="Arne" last="Holmgren">Arne Holmgren</name></author><author><name sortKey="Brismar, Kerstin" sort="Brismar, Kerstin" uniqKey="Brismar K" first="Kerstin" last="Brismar">Kerstin Brismar</name></author><author><name sortKey="Ungerstedt, Johanna S" sort="Ungerstedt, Johanna S" uniqKey="Ungerstedt J" first="Johanna S" last="Ungerstedt">Johanna S. Ungerstedt</name></author></analytic><series><title level="j">Acta diabetologica</title><idno type="eISSN">1432-5233</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Diabetes Mellitus, Type 2 (blood)</term><term>Female (MeSH)</term><term>Glucose Intolerance (blood)</term><term>Glucose Tolerance Test (MeSH)</term><term>Glutaredoxins (blood)</term><term>Humans (MeSH)</term><term>Male (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxidative Stress (physiology)</term><term>Spectrometry, Fluorescence (MeSH)</term><term>Statistics, Nonparametric (MeSH)</term><term>Young Adult (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term><term>Diabète de type 2 (sang)</term><term>Femelle (MeSH)</term><term>Glutarédoxines (sang)</term><term>Humains (MeSH)</term><term>Hyperglycémie provoquée (MeSH)</term><term>Intolérance au glucose (sang)</term><term>Jeune adulte (MeSH)</term><term>Mâle (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Spectrométrie de fluorescence (MeSH)</term><term>Statistique non paramétrique (MeSH)</term><term>Stress oxydatif (physiologie)</term><term>Technique de Western (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Glutaredoxins</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Diabetes Mellitus, Type 2</term><term>Glucose Intolerance</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Stress oxydatif</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Diabète de type 2</term><term>Glutarédoxines</term><term>Intolérance au glucose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Blotting, Western</term><term>Female</term><term>Glucose Tolerance Test</term><term>Humans</term><term>Male</term><term>Oxidation-Reduction</term><term>Spectrometry, Fluorescence</term><term>Statistics, Nonparametric</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Femelle</term><term>Humains</term><term>Hyperglycémie provoquée</term><term>Jeune adulte</term><term>Mâle</term><term>Oxydoréduction</term><term>Spectrométrie de fluorescence</term><term>Statistique non paramétrique</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Oxidative stress induced by hyperglycemia is a key factor in the pathogenesis of diabetes complications. Glutaredoxin 1(Grx1) is a cytosolic redox protein that catalyzes GSH-dependent thiol redox reactions and reversible protein S-glutathionylation. In humans, Grx1 antigen has previously been detected in plasma; however, it has hitherto been unclear if plasma Grx1 is enzymatically active, which would indicate an extracellular function of the protein. Given that glucose overload damages cells through oxidative stress responses, we investigated whether postprandial hyperglycemia induces changes in extracellular Grx1 in patients with abnormal glucose tolerance and healthy subjects. Using a novel sensitive fluorescent substrate assay, we demonstrated that plasma Grx consists of active protein. Grx antigen, activity and total antioxidant capacity were significantly elevated in patients compared to healthy subjects. In response to oral glucose tolerance test, Grx activity and antioxidant capacity increased significantly in healthy volunteers, however, not to the high levels of the patients. In conclusion, these results indicate an extracellular function of plasma Grx in blood glucose metabolism. Thus, Grx may be a marker of increased oxidative stress during hyperglycemia in healthy subjects and may be a risk marker of progression toward diabetes onset. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23836328</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2014</Year><Month>03</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-5233</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Acta diabetologica</Title><ISOAbbreviation>Acta Diabetol</ISOAbbreviation></Journal><ArticleTitle>Plasma glutaredoxin activity in healthy subjects and patients with abnormal glucose levels or overt type 2 diabetes.</ArticleTitle><Pagination><MedlinePgn>225-32</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00592-013-0498-2</ELocationID><Abstract><AbstractText>Oxidative stress induced by hyperglycemia is a key factor in the pathogenesis of diabetes complications. Glutaredoxin 1(Grx1) is a cytosolic redox protein that catalyzes GSH-dependent thiol redox reactions and reversible protein S-glutathionylation. In humans, Grx1 antigen has previously been detected in plasma; however, it has hitherto been unclear if plasma Grx1 is enzymatically active, which would indicate an extracellular function of the protein. Given that glucose overload damages cells through oxidative stress responses, we investigated whether postprandial hyperglycemia induces changes in extracellular Grx1 in patients with abnormal glucose tolerance and healthy subjects. Using a novel sensitive fluorescent substrate assay, we demonstrated that plasma Grx consists of active protein. Grx antigen, activity and total antioxidant capacity were significantly elevated in patients compared to healthy subjects. In response to oral glucose tolerance test, Grx activity and antioxidant capacity increased significantly in healthy volunteers, however, not to the high levels of the patients. In conclusion, these results indicate an extracellular function of plasma Grx in blood glucose metabolism. Thus, Grx may be a marker of increased oxidative stress during hyperglycemia in healthy subjects and may be a risk marker of progression toward diabetes onset. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Yatao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Huihui</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Montano</LastName><ForeName>Sergio</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hegestam</LastName><ForeName>Jesper</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ekberg</LastName><ForeName>Neda Rajamand</ForeName><Initials>NR</Initials></Author><Author ValidYN="Y"><LastName>Holmgren</LastName><ForeName>Arne</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Brismar</LastName><ForeName>Kerstin</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ungerstedt</LastName><ForeName>Johanna S</ForeName><Initials>JS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>07</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Acta Diabetol</MedlineTA><NlmUniqueID>9200299</NlmUniqueID><ISSNLinking>0940-5429</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003924" MajorTopicYN="N">Diabetes Mellitus, Type 2</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018149" MajorTopicYN="N">Glucose Intolerance</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005951" MajorTopicYN="N">Glucose Tolerance Test</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013050" MajorTopicYN="N">Spectrometry, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018709" MajorTopicYN="N">Statistics, Nonparametric</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>02</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>06</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23836328</ArticleId><ArticleId IdType="doi">10.1007/s00592-013-0498-2</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><noCountry><name sortKey="Brismar, Kerstin" sort="Brismar, Kerstin" uniqKey="Brismar K" first="Kerstin" last="Brismar">Kerstin Brismar</name><name sortKey="Ekberg, Neda Rajamand" sort="Ekberg, Neda Rajamand" uniqKey="Ekberg N" first="Neda Rajamand" last="Ekberg">Neda Rajamand Ekberg</name><name sortKey="Hegestam, Jesper" sort="Hegestam, Jesper" uniqKey="Hegestam J" first="Jesper" last="Hegestam">Jesper Hegestam</name><name sortKey="Holmgren, Arne" sort="Holmgren, Arne" uniqKey="Holmgren A" first="Arne" last="Holmgren">Arne Holmgren</name><name sortKey="Montano, Sergio" sort="Montano, Sergio" uniqKey="Montano S" first="Sergio" last="Montano">Sergio Montano</name><name sortKey="Ungerstedt, Johanna S" sort="Ungerstedt, Johanna S" uniqKey="Ungerstedt J" first="Johanna S" last="Ungerstedt">Johanna S. Ungerstedt</name><name sortKey="Zhang, Huihui" sort="Zhang, Huihui" uniqKey="Zhang H" first="Huihui" last="Zhang">Huihui Zhang</name></noCountry><country name="Suède"><region name="Svealand"><name sortKey="Du, Yatao" sort="Du, Yatao" uniqKey="Du Y" first="Yatao" last="Du">Yatao Du</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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